Subsequently, the cultures of each time point were prepared for flow cytometry as described before [ 3 , 12 ].
For each sample, the green fluorescent signals of 50, cells were collected by a FITC filter. The fluorescent intensity was calculated in Arbitrary Units AU. In parallel, the above-described cultures of each time point were also prepared for fluorescence microscopy and applied to agarose slides as described before [ 25 ]. The engineering of gene regulatory networks. Annu Rev Biomed Eng. Kearns DB, Losick R. Cell population heterogeneity during growth of Bacillus subtilis. Genes Dev.
Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis.
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Imaging Fluorescent Proteins
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New York: Humana Press; Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translational fusion in bacteria, yeast and plant cells. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Nat Biotechnol.
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