Green Fluorescent Protein: Properties, Applications, and Protocols, Volume 47, Second Edition

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Subsequently, the cultures of each time point were prepared for flow cytometry as described before [ 3 , 12 ].

Fluorescence Fundamentals

For each sample, the green fluorescent signals of 50, cells were collected by a FITC filter. The fluorescent intensity was calculated in Arbitrary Units AU. In parallel, the above-described cultures of each time point were also prepared for fluorescence microscopy and applied to agarose slides as described before [ 25 ]. The engineering of gene regulatory networks. Annu Rev Biomed Eng. Kearns DB, Losick R. Cell population heterogeneity during growth of Bacillus subtilis. Genes Dev.

Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis.

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Mol Microbiol. Longo D, Hasty J. Dynamics of single-cell gene expression. Mol Syst Biol. Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media. J Appl Microbiol. Small regulatory RNA-induced growth rate heterogeneity of Bacillus subtilis.

Flow cytometry - Wikipedia

PLoS Genet. Higgins D, Dworkin J. Recent progress in Bacillus subtilis sporulation. Cannibalism by sporulating bacteria.

Imaging Fluorescent Proteins

Chen I, Dubnau D. DNA uptake during bacterial transformation. Nat Rev Microbiol. Shank EA, Kolter R. Extracellular signaling and multicellularity in Bacillus subtilis. Curr Opin Microbiol. Sticking together: building a biofilm the Bacillus subtilis way. Stripping Bacillus : ComK auto-stimulation is responsible for the bistable response in competence development.

Transient heterogeneity in extracellular protease production by Bacillus subtilis. Heterochronic phosphorelay gene expression as a source of heterogeneity in Bacillus subtilis spore formation. J Bacteriol. Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation. Environ Microbiol. Tunability and noise dependence in differentiation dynamics. Intrinsic and extrinsic contributions to stochasticity in gene expression. Use of Escherichia coli E.

Book Chapters

In: Murray EJ, editor. Gene transfer and expression protocols.

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  • New York: Humana Press; Active bacterial luciferase from a fused gene: expression of a Vibrio harveyi luxAB translational fusion in bacteria, yeast and plant cells. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Nat Biotechnol.

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    Green fluorescent protein as a marker for gene expression. A guide to choosing fluorescent proteins. Nat Methods.

    Haseloff J, Siemering KR. The uses of green fluorescent protein in plants. Green fluorescent protein: properties, applications, and protocols. Hoboken: Wiley; The dynamic microbe: green fluorescent protein brings bacteria to light. Visualization of differential gene expression by improved cyan fluorescent protein and yellow fluorescent protein production in Bacillus subtilis. Appl Environ Microbiol. Single-cell analysis of Bacillus subtilis biofilms using fluorescence microscopy and flow cytometry. J Vis Exp. Balanced transcription of cell division genes in Bacillus subtilis as revealed by single cell analysis.

    Marston AL, Errington J. Dynamic movement of the ParA-like Soj protein of B. Mol Cell. Dynamic localization of membrane proteins in Bacillus subtilis. Doubrovinski K, Howard M. Stochastic model for Soj relocation dynamics in Bacillus subtilis. Benchmarking various green fluorescent protein variants in Bacillus subtilis , Streptococcus pneumoniae , and Lactococcus lactis for live cell imaging. Sonenshein AL. Control of key metabolic intersections in Bacillus subtilis.

    Boosting heterologous protein production yield by adjusting global nitrogen and carbon metabolic regulatory networks in Bacillus subtilis. Visit our sister site spectroscopyNOW. Banner Ad. Copyright Information Interested in spectroscopy? All Rights Reserved. Email or Customer ID. Forgot password? Old Password. New Password.

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